Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HANKS BALANCED SALT SOLUTION
NC3660344 HANKS BALANCED SALT SOLUTION
Boston Bioproducts Inc Tris-Glycine-SDS Running Buffer (10X) - 1L
The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.
Fd Neurotechnologies Inc PHOSPHATE-BUFFERED 4% PARAFORM
This 1000ml filtered and ready-to-use tissue fixative is freshly made from high purity paraformaldehyde powder. This solution may be stored at room temperature for at least one year without change of the original quality. The standard solution contains 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Other concentrations can also be provided upon request.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 1KG
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 1KG
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt 99.5 tit1KG
HEPES sodium salt 99.5 tit1KG
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 titration25KG
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences PIPES 99 titration1KG
PIPES is a member of the ethanesulfonic acid buffer series first introduced by Good et al. developed to meet certain criteria midrange pKa maximum water solubility and minimum solubility in all other solvents minimal salt effects minimal change in pKa with temperature chemically and enzymatically stable minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 C is near physiological pH PIPES has applications in cell culture work.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Enzo Life Sciences PBS (endotoxin-free) (sterile) (10ml)
Sterile and endotoxin-free PBS for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
Kd Medical Inc TRIS GLYC SDS BUFFER, 10X, 4L
10X Tris Glycine SDS is used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. One 4 liter cubitainer.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML