Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova SM Buffer. 500mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1.5M Tris-HCl, pH 8.8. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 20X PBS Solution. 1000mL, Sterile.
2.7M Sodium Chloride, 54mM Potassium Chloride, 86mM Disodium Phosphate, 28mM Monopotassium Phosphate
Sigma Aldrich Fine Chemicals Biosciences HEPES >=99.5% (titration) | 7365-45-9 | MFCD00006158 | 5KG
HEPES >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 5KG
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate BioUltra, for molecular biology, >=99.5% (T) | 145224-94-8 | MFCD00149409 | 50G
MES monohydrate BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 50G
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Sigma Aldrich Fine Chemicals Biosciences MOPS >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 25G
MOPS >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 25G
Growcells PBS 1X 10L EA
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 1kg
BIS-TRIS | Purity: ≥98.0% (titration) | MW:209.24 | 6976-37-0 | MFCD00002853 | 1kg
Research Products International Corp MES, Anhydrous, 250 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 1kg
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culturePurity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 1kg
Sigma Aldrich Fine Chemicals Biosciences Tricine BioPerformance Certified, suitable for cell culture, >=99% (titration) | 5704-04-1 | MFCD00004277 | 25G
Tricine BioPerformance Certified, suitable for cell culture, >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 25G
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 10G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 10G